Digital pathology images are of a highly heterogeneous nature due to a number of factors: tissue morphology; region-of-interest heterogeneity; variations in the preparation of pathology samples; the optical scanning system; the staining protocol; and even rips or tears in the tissue. Definiens software solutions are designed to address these challenges and facilitate consistent detection of regions of interest in the tissue, as well as in cellular, and even sub-cellular components – resulting in robust, automated quantification.