Customer Testimonials

Brian Lockhart

Director Molecular Pharmacology and Pathophysiology
Servier
In order to put molecular histopathology at the core of Servier’s drug discovery programs, the decision to integrate Definiens’ innovative and robust Definiens Enterprise Image Intelligence™ Suite is a critical step in the success of this strategy.

Dr. Elke Persohn

Head of the Cellular Imaging Group
Novartis Pharma AG
Definiens solved our problem of automatically detecting small intestine crypts and counting proliferating and non-proliferating cells which was not possible with any other software solution.
RELATED RESOURCE
TITLE:Automated Analysis of Cell Proliferation in Images of BrdU Stained Rat Cecum and Colon

Dr. Elena Di Daniel

Senior Scientist
GlaxoSmithKline
Image analysis is frequently a bottleneck when evaluation morphological endpoints in cellular assays. Using Definiens automated process the time to completion of analysis is significantly reduced, coupled with a significant increase in objectivity. Definiens’ technology is now integral in the analysis of the growth cone collapse assay at GlaxoSmithKline.
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TITLE:Complex image-based cell biology endpoint analysis for drug discovery using Definiens software

Pramila Ramani

Consultant Pathologist
University of Bristol
I found Definiens’ support for Tissue Studio®, including web sessions, excellent. The software is very user-friendly. The staff is very helpful and respond promptly; the issues are always resolved in a satisfactory manner.

Sebastian Brandner

Professor of Neuropathology
UCL Institute of Neurology
Our first digital pathology analysis with Definiens Tissue Studio® addressed referees' criticism after submitting the publication of a brain tumour mouse model for peer review. In this model we had generated brain tumours and had characterised them by immunhistochemistry for neural stem cell markers. Our first submission included a semiquantitative assessment of marker intensity and extent on the experimental brain tumours. The referees requested to quantitate expression levels, to complement our cDNA MicroArray expression data.

The generation of digital data for a large number of experimental brain tumours strengthened our statements and allowed us to correlate the expression with the MicroArray data.

Resubmission of the publication (to a different, equally rated Journal) resulted in nearly immediate acceptance. I believe that the addition of fully quantitative, objective data contributed significantly to this.

In the meantime, we have used Definiens Tissue Studio regularly for this type of analysis. One of the positive effects is the retention of the quantification algorithm and reuse of existing data for future comparative studies.

RELATED RESOURCE
TITLE:Comparative Expression Analysis Reveals Lineage Relationships between Human and Murine Gliomas and a Dominance of Glial Signatures during Tumor Propagation In Vitro
PUBLICATION: Cancer Res; 73(18); 5834–44

Steve Bagley

Head of Advanced Imaging and Cytometry
Cancer Research UK: Manchester Institute
My laboratory has been providing whole slide histological analysis since 2008 and has been utilising the Definiens software suite since 2011. Routinely my team visualise and take through to analysis over 1000 slides a week from translational sources (for example patients, patient derived xenographs) and fundamental research. Our remit covers the study of cancer to have real patient benefit in a variety of tissues which include lung, prostate, skin, and pancreas.

Definiens is used in studies such as tumour/stroma interactions, tumour hypoxia, the evolution of the tumour in response to treatment, identification and characterisation of biomarkers, and drug discovery applications across fourteeen laboratories with a user base of around 65 MPhil and PhD candidates, post-doctorial researchers and staff scientists. Certainly our work with large scale tissue micro arrays would be difficult without the Definiens suite.

The software is utilised with the output from four slide scanners, three whole slide (IHC and fluorescence) and one spectrophotometric system. In addition to our translational work aided by Definiens our current research endeavors include applying Definiens to associate histology and its analysis with other data sources such as MR, raman, ultrasound, and photo-acoustics.

The robustness of the Definiens suite has real patient benefit in GLP regulated studies in addition to being a development tool in fundamental studies.

On site we train our scientists in the use of Definiens, and also offer a consultancy service for the research groups for advanced use. Training our researchers in image analysis and data mining has benefit for their research endeavors, but also in attained skills are valuable on the CVs.