Dr. Elke Persohn
Dr. Elena Di Daniel
The generation of digital data for a large number of experimental brain tumours strengthened our statements and allowed us to correlate the expression with the MicroArray data.
Resubmission of the publication (to a different, equally rated Journal) resulted in nearly immediate acceptance. I believe that the addition of fully quantitative, objective data contributed significantly to this.
In the meantime, we have used Definiens Tissue Studio regularly for this type of analysis. One of the positive effects is the retention of the quantification algorithm and reuse of existing data for future comparative studies.
Definiens is used in studies such as tumour/stroma interactions, tumour hypoxia, the evolution of the tumour in response to treatment, identification and characterisation of biomarkers, and drug discovery applications across fourteeen laboratories with a user base of around 65 MPhil and PhD candidates, post-doctorial researchers and staff scientists. Certainly our work with large scale tissue micro arrays would be difficult without the Definiens suite.
The software is utilised with the output from four slide scanners, three whole slide (IHC and fluorescence) and one spectrophotometric system. In addition to our translational work aided by Definiens our current research endeavors include applying Definiens to associate histology and its analysis with other data sources such as MR, raman, ultrasound, and photo-acoustics.
The robustness of the Definiens suite has real patient benefit in GLP regulated studies in addition to being a development tool in fundamental studies.On site we train our scientists in the use of Definiens, and also offer a consultancy service for the research groups for advanced use. Training our researchers in image analysis and data mining has benefit for their research endeavors, but also in attained skills are valuable on the CVs.