Sep 9, 2017
Multidimensional phenotyping of breast cancer cell lines to guide preclinical research
J. M. Saunus, C. E. Smart, J. R. Kutasovic, R. L. Johnston, P. Kalita-de Croft, M. Miranda, E. N. Rozali, A. C. Vargas, L. E. Reid, E. Lorsy, S. Cocciardi, T. Seidens, A. E. McCart Reed, A. J. Dalley, L. F. Wockner, J. Johnson, D. Sarkar, M. E. Askarian-Amiri, P. T. Simpson, K. K. Khanna, G. Chenevix-Trench, F. Al-Ejeh, S. R. Lakhani , Breast Cancer Research and Treatment , DOI: 10.1007/s10549-017-4496-x
Purpose: Cell lines are extremely useful tools in breast cancer research. Their key benefits include a high degree of control over experimental variables and reproducibility. However, the advantages must be balanced against the limitations of modelling such a complex disease in vitro. Informed selection of cell line(s) for a given...
Sep 5, 2017
Linc-ing Circulating Long Non-coding RNAs to the Diagnosis and Malignant Prediction of Intraductal Papillary Mucinous Neoplasms of the Pancreas
J. B. Permuth, D.-T. Chen, S. J. Yoder, J. Li, A. T. Smith, J. W. Choi, J. Kim, Y. Balagurunathan, K. Jiang, D. Coppola, B. A. Centeno, J. Klapman, P. Hodul, F. A. Karreth, J. G. Trevino, N. Merchant, A. Magliocco, M. P. Malafa, R. Gillies , Scientific Reports , DOI: 10.1038/s41598-017-09754-5
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease that lacks effective biomarkers for early detection. We hypothesized that circulating long non-coding RNAs (lncRNAs) may act as diagnostic markers of incidentally-detected cystic PDAC precursors known as intraductal papillary mucinous neoplasms (IPMNs) and predictors of their pathology/histological classification. Using NanoString nCounter® technology,...
Aug 25, 2017
Comprehensive Intrametastatic Immune Quantification and Major Impact of Immunoscore on Survival
B. Mlecnik, M. Van den Eynde, G. Bindea, S. E. Church, A. Vasaturo, T. Fredriksen, L. Lafontaine, N. Haicheur, F. Marliot, D. Debetancourt, G. Pairet, A. Jouret-Mourin, J.-F. Gigot, C. Hubert, E. Danse, C. Dragean J. Carrasco, Y. Humblet, V. Valge-Archer, A. Berger, F. Pagès, J.-P. Machiels, J. Galon , Journal of the National Cancer Institute , DOI: 10.1093/jnci/djx123
Background: This study assesses how the metastatic immune landscape is impacting the response to treatment and the outcome of colorectal cancer (CRC) patients.Methods: Complete curative resection of metastases (n = 441) was performed for two patient cohorts (n = 153). Immune densities were quantified in the center and invasive margin of all metastases. Immunoscore...
Aug 24, 2017
The Natural Fungal Metabolite Beauvericin Exerts Anticancer Activity In Vivo: A Pre-Clinical Pilot Study
D. Heilos, Y. Rodríguez-Carrasco, B. Englinger, G. Timelthaler, S. van Schoonhoven, M. Sulyok, S. Boecker, R. D. Süssmuth, P. Heffeter, R. Lemmens-Gruber, R. Dornetshuber-Fleiss, W. Berger , Toxins , DOI: 10.3390/toxins9090258
Recently, in vitro anti-cancer properties of beauvericin, a fungal metabolite were shown in various cancer cell lines. In this study, we assessed the specificity of this effect by comparing beauvericin cytotoxicity in malignant versus non-malignant cells. Moreover, we tested in vivo anticancer effects of beauvericin by treating BALB/c and CB-17/SCID...
Aug 22, 2017
Human Pancreatic Tumors Grown in Mice Release Tissue Factor-positive Microvesicles that Increase Venous Clot Size
Y. Hisada, C. Ay, A.C. Auriemma, B.C. Cooley, N. Mackman , Journal of Thrombosis and Haemostatis , DOI: 10.1111/jth.13809
Background: Pancreatic cancer patients have a high rate of venous thromboembolism. Human pancreatic tumors and cell lines express high levels of tissue factor (TF) and release TF-positive microvesicles (TF+MVs). In pancreatic cancer patients, tumor-derived TF+MVs are present in blood and increased levels are associated with venous thromboembolism and decreased survival....