Aug 18, 2017
Impaired periodontium and temporomandibular joints in TNF-α transgenic mice
T. Dobsak, P. Heimel, S. Tangl, U. Schwarze, G. Schett, R. Gruber , Journal of Clinical Periodontology , DOI: 10.1111/jcpe.12799
Aim: TNF-α is a pathological factor causing the characteristic symptoms of periodontal disease and rheumatoid arthritis. In this study we describe the phenotypes of human tumor necrosis factor (TNF)-α transgenic mice (hTNFtg) with respect to their periodontium and the temporomandibular joint (TMJ).Material and methods: Periodontal structures, TMJ, and skull shape...
Aug 17, 2017
Interferon-γ-Driven iNOS: A Molecular Pathway to Terminal Shock in Arenavirus Hemorrhagic Fever
M. M. Remy, M. Sahin, L. Flatz, T. Regen, L. Xu, M. Kreutzfeldt, B. Fallet, C. Doras, T. Rieger, L. Bestmann, U.-K. Hanisch, B. A. Kaufmann, D. Merkler, D. D. Pinschewer , Cell Host & Microbe , DOI: 10.1016/j.chom.2017.07.008
Arenaviruses such as Lassa virus (LASV) cause hemorrhagic fever. Terminal shock is associated with a systemic cytokine storm, but the mechanisms are ill defined. Here we used HLA-A2-expressing mice infected with a monkey-pathogenic strain of lymphocytic choriomeningitis virus (LCMV-WE), a close relative of LASV, to investigate the pathophysiology of arenavirus...
Aug 14, 2017
IHC-based subcellular quantification provides new insights into prognostic relevance of FLIP and procaspase-8 in non-small-cell lung cancer
R. A. Hutchinson, H. G. Coleman, K. Gately, V. Young, S. Nicholson, R. Cummins, E. Kay, S. O. Hynes, P. D. Dunne, S. Senevirathne, P. W. Hamilton, D. G. McArt, D. B. Longley , Cell Death Discovery , DOI:10.1038/cddiscovery.2017.50
In this study, we developed an image analysis algorithm for quantification of two potential apoptotic biomarkers in non-small-cell lung cancer (NSCLC): FLIP and procaspase-8. Immunohistochemical expression of FLIP and procaspase-8 in 184 NSCLC tumors were assessed. Individual patient cores were segmented and classified as tumor and stroma using the Definiens...
Aug 8, 2017
Assessment of Ki67 expression for breast cancer subtype classification and prognosis in the Nurses’ Health Study
M. A. Healey, K. A. Hirko, A. H. Beck, L. C. Collins S. J. Schnitt, A. H. Eliassen, M.D. Holmes, R. M. Tamimi, A. Hazra , Breast Cancer Research and Treatment , DOI: 10.1007/s10549-017-4421-3
Purpose: Ki67 is a proliferation marker commonly assessed by immunohistochemistry in breast cancer, and it has been proposed as a clinical marker for subtype classification, prognosis, and prediction of therapeutic response. However, the clinical utility of Ki67 is limited by the lack of consensus on the optimal cut point for...
Aug 4, 2017
Tumor Necrosis Factor (TNF) Bioactivity at the Site of an Acute Cell-Mediated Immune Response Is Preserved in Rheumatoid Arthritis Patients Responding to Anti-TNF Therapy
R. Byng-Maddick, C. T. Turner, G. Pollara, M. Ellis, N. J. Guppy, L. C. K. Bell, M. R. Ehrenstein, M. Noursadeghi , frontiers in Immunology , DOI: 10.3389/fimmu.2017.00932
The impact of anti-tumor necrosis factor (TNF) therapies on inducible TNF-dependent activity in humans has never been evaluated in vivo. We aimed to test the hypothesis that patients responding to anti-TNF treatments exhibit attenuated TNF-dependent immune responses at the site of an immune challenge. We developed and validated four context-specific...