Spatially-resolved, Highly Multiplexed (up to 800-plex) Digital Characterization of Protein Distribution and Abundance in FFPE Tissue Sections Using Optical Barcoding
Joseph Beecham, PhD, Senior Vice President R&D, Nonostring Technologies
Intratumoral heterogeneity has emerged as a critical challenge to the implementation of targeted therapeutics. Historically, immunohistochemistry (IHC) has been used to assess spatial heterogeneity of proteins; however, it has been difficult to quantify protein abundance at high multiplex and wide dynamic range. Here, we report the development and validation of...
Optimal Patient Selection for Immune-Therapies of Cancer Using Tissue Phenomics*
Guenter Schmidt, PhD, Vice President Research, Definiens AG
Current immune-therapies for advanced melanoma and lung cancer show impressive response rates and long-term survival. Approximately one of five patient lives is saved by modulating his immune system with the blockade of an immune checkpoint. On the other hand, four of five patients do not respond to a very expensive...
Morphometric-molecular Approaches to Human Diabetic Kidney Disease and Chronic Kidney Disease*
Jeff Hodgin, MD, Assistant Professor of Pathology, University of St. Andrews
The kidney biopsy is an essential component for the diagnosis and effective therapeutic management of patients with kidney disease, however most analyses are qualitative and necessarily descriptive, lacking quantitative measurements and evaluation of underlying molecular mechanisms. Patients with diabetes have a significant risk of developing diabetic kidney disease (DKD) that...
The Tissue Image as an Integrative System*
David Harrison, MD, Professor of Pathology, University of St. Andrews
How fundamental is the implementation of tissue phenomics into practice? One aim of medical advances should be to predict what will happen, rather than simply to describe what has happened; but scientific medicine has often been based on reductionism rather than synthesis. Much of this comes from the historical importance...
Opportunities and Challenges of Bringing Digital Pathology to the Clinic
Anthony M. Magliocco, MD, Chair of Anatomic Pathology and Executive Director of Esoteric Laboratory Services, Moffitt Cancer Center
Tremendous advances have been made is the technological capabilities of digital image analysis which could have significant impact in the clinical pathology laboratory, yet there is very slow adoption of these technologies into routine clinical care. Areas of impact include tele pathology, biomarker analysis, complex functional tissue analysis, education, and...