Mar 13, 2018
Tissue Phenomics for prognostic biomarker discovery in low- and intermediate-risk prostate cancer
N. Harder, M. Athelogou, H. Hessel, N. Brieu, M. Yigitsoy, J. Zimmermann, M. Baatz, A. Buchner, C. G. Stief, T. Kirchner, G. Binnig, G. Schmidt, R. Huss , Scientific Reports , DOI: 10.1038/s41598-018-22564-7
Tissue Phenomics is the discipline of mining tissue images to identify patterns that are related to clinical outcome providing potential prognostic and predictive value. This involves the discovery process from assay development, image analysis, and data mining to the final interpretation and validation of the findings. Importantly, this process is...
Mar 12, 2018
Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer
A. Costa, Y. Kieffer, A. Scholer-Dahirel, F. Pelon, B. Bourachot, M. Cardon, P. Sirven I. Magagna, L. Fuhrmann, C. Bernard, C. Bonneau, M. Kondratova, I. Kuperstein, A. Zinovyev, A.-M. Givel, M.-C. Parrini, V. Soumelis, A. Vincent-Salomon, F. Mechta-Grigoriou , Cancer Cell , DOI: 10.1016/j.ccell.2018.01.011
Carcinoma-associated fibroblasts (CAF) are key players in the tumor microenvironment. Here, we characterize four CAF subsets in breast cancer with distinct properties and levels of activation. Two myofibroblastic subsets (CAF-S1, CAF-S4) accumulate differentially in triple-negative breast cancers (TNBC). CAF-S1 fibroblasts promote an immunosuppressive environment through a multi-step mechanism. By secreting...
Mar 6, 2018
Qualitative and Quantitative Analysis of Cardiac Progenitor Cells in Cases of Myocarditis and Cardiomyopathy
M. Gerisch, J. Smettan, S. Ebert, M. Athelogou, B. Brand-Saberi, N. Spindler, W. C. Mueller, S. Giri, A. Bader , frontiers in Genetics , DOI: 10.3389/fgene.2018.00072
We aimed to identify and quantify CD117+ and CD90+ endogenous cardiac progenitor cells (CPC) in human healthy and diseased hearts. We hypothesize that these cells perform a locally acting, contributing function in overcoming medical conditions of the heart by endogenous means. Human myocardium biopsies were obtained from 23 patients with...
Mar 6, 2018
Measuring multiple parameters of CD8+ tumor-infiltrating lymphocytes in human cancers by image analysis
K. E. Steele, T. H. Tan, R. Korn, K. Dacosta, C. Brown, M. Kuziora, J. Zimmermann, B. Laffin, M. Widmaier, L. Rognoni, R. Cardenes, K. Schneider, A. Boutrin, P. Martin, J. Zha, T. Wiestler , Journal for ImmunoTherapy of Cancer , DOI: 0.1186/s40425-018-0326-x
Background: Immuno-oncology and cancer immunotherapies are areas of intense research. The numbers and locations of CD8+ tumor-infiltrating lymphocytes (TILs) are important measures of the immune response to cancer with prognostic, pharmacodynamic, and predictive potential. We describe the development, validation, and application of advanced image analysis methods to characterize multiple immunohistochemistry-derived...
Mar 6, 2018
Context-based interpolation of coarse deep learning prediction maps for the segmentation of fine structures in immunofluorescence images
N. Brieu, C. G. Gavriel, D. J. Harrison, P. D. Caie, G. Schmidt , SPIE. Digital Library , DOI: 10.1117/12.2292794
The automatic analysis of digital pathology images is becoming of increasing interest for the development of novel therapeutic drugs and of the associated companion diagnostic tests in oncology. A precise quantification of the tumor microenvironment and therefore an accurate segmentation of the tumor extent are critical in this context. In...